Journal: bioRxiv
Article Title: A high-throughput nematode sensory assay reveals an inhibitory effect of ivermectin on parasite gustation
doi: 10.1101/2023.04.25.538347
Figure Lengend Snippet: A schematic overview of the procedures used to produce the data in B-D. C. elegans are washed from NGM culture plates into a 1.5 mL tube and washed 3 times to remove residual debris and bacteria and to enrich for young adult worms. Worms are then mixed with liquid PF-127 and quickly added to the devices to allow for polymerization of the hydrogel. Initial experiments used 24-well devices with 3 slices, and later experiments used the gustatory microplate. Cues and controls are added as liquid bubbles to the top and bottom of the polymer, and then devices are incubated in a humid chamber to allow for worm gustation and burrowing. (B) C. elegans are attracted to either 100 mM or 200 mM NaCl, with a slightly higher CI when the cue was added to the top of the well. (C) Three-layer Stacks devices were separated after 1 hr. incubation, and worms in each slice were counted. Wild type (N2) worms are attracted to 200 mM NaCl and repelled by 10 mM quinine. tax-4(p678) worms show no response to NaCl and are attracted to quinine. (D) Chemotaxis indices of five chemosensory-defective knockout strains of C. elegans in response to 200 mM NaCl and 10 mM quinine. In each figure, dots represent the value for a single well of the device, large red dots represent the mean, and error bars represent the confidence limits. ***: p <= 0.001, ****: p <= 0.0001.
Article Snippet: In addition to this, a 30% w/w PF-127 (Sigma Aldrich)/water gel solution was created 2-3 days before assay date to allow the PF-127 to dissolve completely at 4°C.
Techniques: Incubation, Chemotaxis Assay, Knock-Out
